Experiment02

The Determination of Food Dye in Packaged Soft Drinks
包裝飲品中食物色素的測定

	Apparatus for construction of LED colourimeter: Green LED, Infra-red LED, 3V button battery, Connecting wires, Digital voltmeter, Plastic stand 製作LED比色計所需儀器：綠光LED燈，紅外光LED燈， 3V鈕型電池，電線，電子伏特計，塑膠座
	Plastic stand for holding a cuvette and two LEDs (Produced by 3D printing) 塑膠座用於架著比色皿和兩個LED燈 (透過3D打印而製成)
	Insert a button battery between the leads of the green LED. Secure the battery with Blu-tack. Insert the Green LED into the openings of the plastic stand. 在綠光LED燈的兩端引線間放入鈕型電池。用寶貼牢固LED燈。將LED燈插入塑膠架相對的開口端。
	Connect the two leads of IR LED to voltmeter. 將紅外光LED燈的引線連接伏特計。
	Turn on the voltmeter. 開啟伏特計。
	Stock solution of Sunset Yellow FCF (100 ppm) 配製好的日落黃標準溶液 (100 ppm)
	Prepare four Sunset Yellow FCF standard solutions by pipetting 5.0, 10.0, 15.0 and 20.0 cm³ of the given Sunset Yellow FCF stock solution into four 25 cm³ volumetric flasks respectively. Make up to volume using deionised water. 配製四個日落黃標準溶液，用移液管分別抽取5.0、 10.0、15.0和20.0 cm³的所提供已配好的日落黃標準溶液，加入四個25 cm³ 的容量瓶中，用去離子水加至各容量瓶的刻度。
	Plastic cuvette 塑膠比色皿
	Rinse a cuvette and fill it at least half-full with deionised water. Wipe the outside of the cuvette with a tissue to make sure that the cuvette surfaces are clean and dry. 用去離子水沖洗比色皿後，將去離子水注入比色皿至半滿。用紙巾將比色皿外壁的水抹掉，並確保比色皿的外壁表面乾淨。
	Place the cuvette in the sample compartment of the colorimeter and record the voltmeter reading for the LED detector. Repeat the procedures for the five Sunset Yellow FCF standard solutions you obtained. Rinse the cuvette with the solution to be measured. 將比色皿放置在比色計中，記錄連接LED探測器的萬用電表所得讀數。留意比色皿的光滑面應向著各LED燈。分別用五個日落黃標準溶液重複步驟。每次先用將要量度的溶液沖洗比色皿。
	Packaged soft drink sample 包裝飲品樣本
	Degas the packaged soft drink sample using an ultrasonic bath for 10 minutes, or leave the sample in air for 2 hours before use. 將包裝飲品樣本置於超聲波浴內排氣十分鐘，或在實驗前放置樣本於空氣中兩小時。
	Dilute the soft drink sample by pipetting 5.0 cm³ of the sample solution into 10 cm³ volumetric flask. 用移液管抽取5.0 cm³的樣本溶液，加入10 cm³ 的容量瓶中，以稀釋包裝飲品樣本。
	Make up to volume using deionised water. 用去離子水加至容量瓶的刻度。
	Rinse a cuvette and fill it at least half-full with the diluted sample. Place the cuvette in the sample compartment of the colorimeter and record the voltmeter reading for the LED detector. 用經稀釋的樣本沖洗比色皿後，將稀釋的樣本注入比色皿至半滿。用紙巾將比色皿外壁的水抹掉，並確保比色皿的外壁表面乾淨。

	Apparatus for construction of LED colourimeter: Green LED, Infra-red LED, 3V button battery, Connecting wires, Digital voltmeter, Plastic stand 製作LED比色計所需儀器：綠光LED燈，紅外光LED燈， 3V鈕型電池，電線，電子伏特計，塑膠座
	Plastic stand for holding a cuvette and two LEDs (Produced by 3D printing) 塑膠座用於架著比色皿和兩個LED燈 (透過3D打印而製成)
	Insert a button battery between the leads of the green LED. Secure the battery with Blu-tack. Insert the Green LED into the openings of the plastic stand. 在綠光LED燈的兩端引線間放入鈕型電池。用寶貼牢固LED燈。將LED燈插入塑膠架相對的開口端。
	Connect the two leads of IR LED to voltmeter. 將紅外光LED燈的引線連接伏特計。
	Turn on the voltmeter. 開啟伏特計。
	Stock solution of Sunset Yellow FCF (100 ppm) 配製好的日落黃標準溶液 (100 ppm)
	Prepare four Sunset Yellow FCF standard solutions by pipetting 5.0, 10.0, 15.0 and 20.0 cm³ of the given Sunset Yellow FCF stock solution into four 25 cm³ volumetric flasks respectively. Make up to volume using deionised water. 配製四個日落黃標準溶液，用移液管分別抽取5.0、 10.0、15.0和20.0 cm³的所提供已配好的日落黃標準溶液，加入四個25 cm³ 的容量瓶中，用去離子水加至各容量瓶的刻度。
	Plastic cuvette 塑膠比色皿
	Rinse a cuvette and fill it at least half-full with deionised water. Wipe the outside of the cuvette with a tissue to make sure that the cuvette surfaces are clean and dry. 用去離子水沖洗比色皿後，將去離子水注入比色皿至半滿。用紙巾將比色皿外壁的水抹掉，並確保比色皿的外壁表面乾淨。
	Place the cuvette in the sample compartment of the colorimeter and record the voltmeter reading for the LED detector. Repeat the procedures for the five Sunset Yellow FCF standard solutions you obtained. Rinse the cuvette with the solution to be measured. 將比色皿放置在比色計中，記錄連接LED探測器的萬用電表所得讀數。留意比色皿的光滑面應向著各LED燈。分別用五個日落黃標準溶液重複步驟。每次先用將要量度的溶液沖洗比色皿。
	Packaged soft drink sample 包裝飲品樣本
	Degas the packaged soft drink sample using an ultrasonic bath for 10 minutes, or leave the sample in air for 2 hours before use. 將包裝飲品樣本置於超聲波浴內排氣十分鐘，或在實驗前放置樣本於空氣中兩小時。
	Dilute the soft drink sample by pipetting 5.0 cm³ of the sample solution into 10 cm³ volumetric flask. 用移液管抽取5.0 cm³的樣本溶液，加入10 cm³ 的容量瓶中，以稀釋包裝飲品樣本。
	Make up to volume using deionised water. 用去離子水加至容量瓶的刻度。
	Rinse a cuvette and fill it at least half-full with the diluted sample. Place the cuvette in the sample compartment of the colorimeter and record the voltmeter reading for the LED detector. 用經稀釋的樣本沖洗比色皿後，將稀釋的樣本注入比色皿至半滿。用紙巾將比色皿外壁的水抹掉，並確保比色皿的外壁表面乾淨。