Core Facilities Genome Sequencing Laboratory

Mobile

Service hour:

* non-CUHK users:
Mon-Fri 9:00am-5:30pm
* CUHK users:
Mon-Fri 9:00am-5:30pm
(non-working hours: subjected to advance approval)

Equipments are operated by research assistant and scientific officer, unless otherwise stated.

Scope of Service	Name of Equipment	Location at CSLB of CUHK	Types of Analysis	Types of Service	Service Rate (HKD)
Scope of Service	Name of Equipment	Location at CSLB of CUHK	Types of Analysis	Types of Service	CUHK Internal Users	Other Institutes	External Users
Genome pyrosequencing	Roche GS Titanium	LG211	Shotgun pyrosequencing	Throughput: 400Mbp PTP: Sample QC, library preparation, enrichment titration, sequencing run, data QC, data assembly & delivery	Ask for a quote	Ask for a quote	Ask for a quote
			Amplicon pyrosequencing	Throughput: 400Mbp PTP: Sample QC, library preparation, enrichment titration, sequencing run, data QC, data assembly & delivery	Ask for a quote	Ask for a quote	Ask for a quote
			Transcriptome pyrosequencing	Throughput: 400Mbp PTP: Sample QC, library preparation, enrichment titration, sequencing run, data QC, data assembly & delivery	Ask for a quote	Ask for a quote	Ask for a quote
			Long paired end shotgun pyrosequencing	Throughput: 400Mbp PTP: Sample QC, library preparation, enrichment titration, sequencing run, data QC, data assembly & delivery	Ask for a quote	Ask for a quote	Ask for a quote
			Small RNA pyrosequencing	Throughput: 400Mbp PTP: Sample QC, library preparation, enrichment titration, sequencing run, data QC, data assembly & delivery	Ask for a quote	Ask for a quote	Ask for a quote

Scope of Service	Name of Equipment	Location at CSLB of CUHK	Types of Analysis	Types of Service	Service Rate (HKD)
Scope of Service	Name of Equipment	Location at CSLB of CUHK	Types of Analysis	Types of Service	CUHK Internal Users	Other Institutes	External Users
Gene Expression Analysis &	7500 Fast Real-time PCR system	LG211	Real-time quantification of nucleic acids during PCR amplification	Quantification Assay	$35/hr	200 /session	300 /session
Genotyping	(Rental of machine for particular time slot, reagent,		differentiate a single nucleotide polymorphism of sample using specific probes contain different fluorescent reporter dyes	Allelic Discrimination Assay	$35/hr	200 /session	300 /session
	and labour not included)		determine presence or absence of a specific target sequence in a sample	Plus/Minus Assay	$35/hr	250 /session	300 /session
			High resolution melting analysis	Identification of new variants	$35/hr	200 /session	300 /session
Quantification and quality assessment	Agilent 2100 Bioanalyzer	LG211	analysis of DNA, RNA, protein and cells	1. Agilent RNA 6000 Pico Total RNA kit, reagents, chip and labour included 2. Agilent DNA 1000, 7500 kits, reagents, chip and labour included 3. Agilent High sensitivity DNA kit, reagents, chip and labour included 4. Protein kit, Cell kit. Reagents, chip and labour included	340 / chip of 11 samples 280 / chip of 12 samples 550 / chip of 11 samples On request	370 /chip of 11 samples 300 / chip of 12 samples 600 / chip of 11 samples On request	400 /chip of 11 samples 350 / chip of 12 samples 645 / chip of 11 samples On request
	GE GeneQuant 1300	LG211	spectrophotometer measuring: Nucleic acid concentration Fluorescent dye labelling density of nucleic acid microarray samples Expanded spectrum measurement and quantitation of fluorescent dye labelled proteins, conjugates, and metalloproteins Bradford, BCA, Lowry Assay analysis of protein Cell density measurements General UV-Vis spectrophotometry	Rental of machine for particular time slot, labour not included	$35/hr	100 /hr	150 /hr

Scope of Service	Name of Equipment	Location at CSLB of CUHK	Types of Analysis	Types of Service	Service Rate (HKD)
Scope of Service	Name of Equipment	Location at CSLB of CUHK	Types of Analysis	Types of Service	CUHK Internal Users	Other Institutes	External Users
Particle size analyser	Beckman Multisizer 3	LG211	Provide size and volume distributions in number, volume, surface area and mass, with an overall range of 0.4µm-1200µm	Particle size analysis (Rental of machine, reagent and labour included)	$50/sample	400 /day	500 /day
Random DNA fragments generation	Genemachines HydroShear	LG211	DNA at any concentration in volumes as small as 40μl can be randomly fractured to within a two-fold size distribution	Random DNA fragments generation (Rental of workstation, labour included)	$35/hr	250/ half day session	300/ half day session
Multiple biological samples disruption	Qiagen TissueLyser	LG211	disrupts multiple biological samples through high-speed shaking in plastic tubes with steel, tungsten carbide, or glass beads	Sample disruption (Rental of workstation, labour included)	$35/hr	250/ half day session	300/ half day session
Microarray scanner	NimbleGen MS 200 Microarray scanner	LG211	Microarray scanning	Comparative genomic hybridization (CGH), Chromatin ImmunoPrecipitation-on-chip (ChIP-chip), DNA methylation, and Gene Expression.	Ask for a quote	Ask for a quote	Ask for a quote
DNA Sequencing	ABI PRISM 3110 Genetic Analyser	LG211	DNA sequencing	1. sequencing run 2. purification and sequencing run 3. sequencing reaction, purification and run	18 /sample 22 /sample 28 /sample	20 /sample 25 /sample 35 /sample	20 /sample 25 /sample 35 /sample
Upcoming Service
Automation Workstation	Biomek® 2000 Laboratory Automation Workstation	LG211	Automated liquid handling	Rental of machine, including labour and consumable (tip & plate) Reagent provided by users (Minimum order, for 3 hours session)	200 /session	300/session	400 /session

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	Core Facilities Genome Sequencing Laboratory The Chinese University of Hong Kong

Core Facilities Genome Sequencing LaboratoryThe Chinese University of Hong Kong

Service hour:

Core Facilities
Genome Sequencing Laboratory
The Chinese University of Hong Kong