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Research Interests: Endometrium / Sperm ion channels and receptors
Ion channels have been implicated in a variety of reproductive events, including sperm activation, sperm-egg interaction. However, the role of ion channels in the reproductive tracts, both male and female, remains vastly unknown. Therefore, investigation of ion channels in the reproductive systems is crucial in elucidating the mechanisms underlying different reproductive processes.
Endometrium - the mucous membrane lining the uterusHow does uterine electrolyte and fluid transport take place?
- ion transport mechanisms
- neurohormonal regulation
- ion channel expression and regulation
- electrolyte transport and implantation
Sperm ion channels and receptors
What are their roles in sperm functions?
Since direct electrophysiological study of sperm ion channels is limited by the small size of sperm, oocyte system is used to functionally express sperm ion channels and receptors.
- characterization of ion channels expressed in Xenopus oocytes
- sperm ion channel regulation by epididymis- and egg-released factors
- receptor expression and interaction with ion channels
Currently employed techniques include: oocyte-clamp, patch-clamp, short-circuit current technique, cell culture, RT-PCR, northern and western blot analysis, immunohistochemical techniques. Research projects involve collaboration with institutes outside of Hong Kong.
Honorary Appointments:
Visiting professor, Shanghai Institute of Cell Biology, Chinese Academy of Science
Visiting professor, Fourth Military Medical University
Visiting professor, Jinan University
Awards and Recognitions
1994 Assisted Reproduction Technology/Andrology International Award
1996 Losartan Travel Award for Experimental Biology'96
1997 National Natural Science Award of China
1998 Inclusion in Who's Who in Science and Engineering (Marquis Publisher, 4th Edition)
Selected Publications:
* H.C.Chan and D.J.Nelson (1992). Chloride-dependent cation conductance activated during cellular shrinkage. Science 257: 669-671.
* H.C.Chan, J.Goldstein and D.J.Nelson (1992). Alternate pathways for chloride conductance activation in normal and cystic fibrosis airway epithelial cells. American Journal of Physiology 262: C1273-1283.
* H.C.Chan, M.A.Kaetzel, D.J.Nelson, P.Hazarika and J.R.Dedman (1992). Antibodies against CFTR derived synthetic peptide inhibit anion currents in human colonic cell line T84. Journal of Biological Chemistry 267: 8411-8416.
* H.C. Chan, W.O. Fu, T.S. Zhou, W.P. Wang, Y.L. Shi and P.Y.D. Wong (1997). Cation and anion channels in rat and human spermatozoa. Biochimica Biophysica Acta 1323:117-129.
* H.C. Chan, C.Q. Liu, S.K. Fong, S.H. Law, L.J. Wu, Eddie So, Y.W. Chung, W.H. Ko and P.Y.D. Wong (1997). Regulation of Cl secretion by extracellular ATP in cultured mouse endometrial epithelium. Journal of Membrane Biology 156: 45-52.
* H.C. Chan, S.K. Fong, S.C. So, Y.W. Chung, and P.Y.D. Wong (1997). Stimulation of anion secretion by ß-adrenoceptors in the mouse endometrial epithelium. Journal of Physiology 501: 517-525.
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